In situ sol–gel preparation of porous alumina monoliths for chromatogr

In situ sol–gel preparation of porous alumina monoliths for chromatographic separations of adenosine phosphateswholesale aluminium oxideA method enabling the in situ preparation of porous alumina monoliths within 100 μm i.d. fused silica capillaries has been developed. These monoliths were prepared using the sol–gel process from a mixture consisting of an inorganic aluminum salt, a porogen, an epoxide, and a solvent. We investigated the effects of varying the preparation conditions on the physical characteristics of the monoliths with respect to their potential application in chromatographic separations. The best columns were obtained from a mixture of aluminum chloride hexahydrate, N,N-dimethylformamide, water, ethanol and propylene oxide. Adenosine phosphates were then separated in the optimized column with retention increasing according to number of phosphate functionalities.https://www.hslabrasive.com